Colombian Essential Oil of Ruta graveolens against Nosocomial Antifungal Resistant Candida Strains

Drug resistance in antifungal therapy, a problem unknown until few years ago, is increasingly assuming importance especially immunosuppressed patients and receiving chemotherapy radiotherapy. In the past years, use of essential oils as an approach to improve effectiveness agents reduce levels has been proposed. Our research aimed evaluate activity Colombian rue, Ruta graveolens, oil (REO) against clinical strains Candida albicans, parapsilopsis, glabrata, tropicalis. Data obtained showed that C. tropicalis albicans were most sensitive showing minimum inhibitory concentrations (MIC) 4.1 8.2 µg/mL REO. Time–kill kinetics assay demonstrated REO fungicidal effect fungistatic albicans. addition, amount 40% biofilm formed by was eradicated using after 1 h exposure. The synergistic together with some compounds also investigated. Fractional concentration index (FICI) synergic effects combined amphotericin B. Lead disruption cellular membrane integrity, consequently resulting increased intracellular leakage macromolecules, thus confirming plasma target mode action